Free Access
Issue
J. Chim. Phys.
Volume 96, Number 1, January 1999
Page(s) 143 - 146
DOI https://doi.org/10.1051/jcp:1999122
J. Chim. Phys. Vol. 96, N°1  p. 143-146
DOI: 10.1051/jcp:1999122

Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

Pouget J.-P.1, Ravanat J.-L.1, Douki T.1, Richard M.-J.2 and Cadet J.1

1  Département de Recherche Fondamentale sur la Matière Condensée, SCIB/Laboratoire “Lésions des Acides Nucléiques", CEA/Grenoble, F-38054 Grenoble Cedex 9, France
2  LBSO/LCR7 No. 8M, Faculté de Pharmacie, Université Joseph Fourier, F-38700 La Tronche, France


Abstract
We used the comet assay associated with DNA-glycosylases to estimate DNA damage in cells exposed to gamma irradiation or photosensitized either with methylene blue or orange acridine. A calibration performed using irradiation allowed the measurement of the steady-state level and the yield of 8-oxodGuo as well as strand breaks and alkali-labile sites.


Résumé
Nous avons utilisé la méthode des comètes associée à des ADN-glycosylases, pour estimer les dommages de l'ADN dans des cellules après l'exposition à un rayonnement gamma ou après photosensibilisation par le bleu de méthylène ou l'acridine orange. Une calibration de la méthode des comètes a permis de mesurer le niveau basal et les taux de formation de 8-oxodGuo ainsi que le nombre de cassures de brins et de sites alcali labiles.


Key words: $\gamma$ irradiation, DNA damages, photosensitization, comet assay


© EDP Sciences 1999

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