Free Access
Issue
J. Chim. Phys.
Volume 96, Number 9/10, October/November/December 1999
Page(s) 1585 - 1594
DOI https://doi.org/10.1051/jcp:1999236
DOI: 10.1051/jcp:1999236


J. Chim. Phys. Vol. 96, N°9/10  p. 1585-1594

Backbone dynamics study of C12A-p8 $^{\it MTCP1}$ using the linear correlation approach at two magnetic field strengths. Effect of protein aggregation.

P. Barthe1, N. Declerck1, M.-A. Delsuc1, J.-F. Lefevre2 and C. Roumestand1

1  Centre de Biochimie Structurale, UMR 9955 du CNRS, INSERM-U414, Université de Montpellier I, Faculté de Pharmacie, 15 avenue Charles Flahault, 34060 Montpellier Cedex 1, France
2  ESBS, Université Louis Pasteur, UPR 9003 du CNRS, boulevard S. Brant, 67400 Illkirch-Graffenstaden, France

Abstract
We discussed here the effect of the presence of non-specific oligomerization states on the backbone dynamics of C12A-p8 $^{\it MTCP1}$. Dynamic parameters were obtained from the analysis of 15N spin relaxation measurements (T 1, T 2 and 15N{H}NOEs) at two magnetic field strengths and on two different protein samples (4 mM and 400  $\mu$M) using the linear correlation assumption between J(0) and J( $\omega$). At high concentration, the presence of aggregation states leads to a significantly higher value for the overall tumbling as compared to the dilute sample, whereas the features of the internal motion are essentially preserved.

Résumé
Nous avons analysé les effets de l'association non spécifique en solution concentrée de la protéine C12A-p8 $^{\it MTCP1}$ sur la dynamique interne de son squelette peptidique. Les informations dynamiques ont été extraites de l'analyse des paramètres de relaxation hétéronucléaire 15N(T 1, T 2 et 15N{H}NOEs) obtenus à deux champs magnétiques et sur deux échantillons (4 mM et 400 $\mu$M) par la méthode dite des corrélations linéaires entre J(0) and J( $\omega$). A haute concentration, la présence d'agrégation entraîne une augmentation significative du temps de réorientation global, par rapport à l'échantillon dilué, mais affecte peu la dynamique interne de la protéine.


Key words: 15N Relaxation - Protein Backbone Dynamics - Aggregation States.
Contents

© EDP Sciences 1999

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