| Issue |
J. Chim. Phys.
Volume 92, 1995
|
|
|---|---|---|
| Page(s) | 1793 - 1796 | |
| DOI | https://doi.org/10.1051/jcp/1995921793 | |
| Published online | 29 May 2017 | |
Quantitative 1H NMR spectroscopy analysis of the poly(3-hydroxyalkanoate) extracted from Rhizobium meliloti cells
1
Laboratoire de génie cellulaire, faculté des sciences, université de Picardie Jules-Verne, 33, rue Saint-Leu, 80039 Amiens Cedex France.
2
Laboratoire de biotechnologie microbienne, IUT, université de Picardie Jules-Verne, av des Facultés Le Bailly, 80025 Amiens Cedex France.
1H NMR analysis was carried out to determine the nature and the concentration of the poly(3-hydroxyalkanoate) (PHA) accumulated by Rhizobium meliloti M5N1. The PHA was identified as being poly(3-hydroxybutyrate) (PHB). Benzene was shown to meet ah the requirements of an internal reference for PHB quantification. NMR data were in good agreement with corresponding data obtained by Gas Chromatography (GC) analysis.
Résumé
La spectroscopie de RMN du proton a permis l’identification et la quantification du PHA extrait des cellules de Rhizobium meliloti MsN1.Sa quantification est mise au point en utilisant le benzène comme référence. Les résultats sont en accord avec ceux obtenus par analyse CPG.
Key words: Rhizobium meliloti / Poly(3-hydroxybutyrate) / 1H NMR and GC quantification
© Elsevier, Paris, 1995