Issue |
J. Chim. Phys.
Volume 89, 1992
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Page(s) | 175 - 181 | |
DOI | https://doi.org/10.1051/jcp/1992890175 | |
Published online | 29 May 2017 |
Vasoactive intestinal contractor (VIC) peptide structure in solution as determinated by proton NMR
1 CCIPE, rue de la Cardonille, 34094 Montpellier Cedex 5, France ;
2 Centre d’Etudes Nucléaires de Saclay, SCM 91191 Gif-sur-Yvette, France.
The Vasoactive Intestinal Contractor peptide has been synthesized and studied by 1H NMR in 50/50 acetonitrile/water mixture. All spin systems were identified and assigned with the aid of 2D experiments. Modeling was performed with constraints obtained by NOEs. Some distances used in modeling were calculated from build-up data. Kinetic exchange of the amide protons showed that the 9-15 helical segment is stabilized by at least three hydrogen bonds. The proposed structure is compared with those of Nle-7 endothelin and Sarafotoxin-S6b previously obtained in same conditions.
Résumé
Le peptide Vasoactive Intestinal Contractor a été synthétisé et étudié par RMN du proton dans le mélange acélonitrile/eau 50/50. Tous les systèmes de spin ont été identifiés à l’aide d’expériences 2D. Certaines distances utilisées pour la modélisation ont été calculées à partir des vitesses d’apparition du NOE. L’échange des protons amides a permis de montrer que trois liaisons hydrogène stabilisent l’hélice 9-15. La structure proposée est comparée à celles obtenues dans les mêmes conditions pour la Nle-7 endothéline et la sarafotoxine-S6b.
© Elsevier, Paris, 1992