Free Access
J. Chim. Phys.
Volume 96, Number 1, January 1999
Page(s) 124 - 131
J. Chim. Phys. Vol. 96, N°1  p. 124-131
DOI: 10.1051/jcp:1999119

Cellular effects produced by internal contamination of rats with tritiated water

Savu D., Moisoi N. and Petcu I.

Institute of Physics and Nuclear Engineering, Department of experimental Physics P.O. Box MG-6, R-76900, Magurele-Bucharest, ROMANIA

The activity of the erythrocyte glucose-6-phosphate dehydrogenase (G6PDH) and the thymidine (TdR) incorporation in the bone marrow cells have been investigated in rats internally contaminated with tritiated water (HTO). The total doses (4.2 cGy; 19.4 cGy and respectively 5 cGy; 20 cGy) were delivered as chronic (100 days) and acute (5 days) contamination. It was observed a significant increase of Glucose-6-Phosphate- Dehydrogenase (G6PDH) activity for 4.2 cGy (0.4 mGy/day). Both types of contamination produced the reduced incorporation of TdR in the bone marrow cells. These results would allow us to speculate that the reduction of the TdR incorporation could be determined by the changes at the level of membrane transport functions for the lower doses (4.2 cGy; 5 cGy), and by radiation induced phenomena at the intracellular level for the larger doses (19.4 cGy; 20 cGy).

Nous avons etudié les effets de l'irradiation des rats par contamination interne à l'eau tritiée, sur l'activité enzymatique de glucose-6-phosphate deshydrogénase (G6PDH) érytrocytaire et sur l'incorporation de thymidine (TdR) dans des cellules de la moelle osseuse. Les doses accumulées par contamination chronique (100 jours) sont 4.2 cGy et 19.4 cGy et par contamination aiguë (5 jours) sont 5 cGy et 20 cGy. Nous avons observé une augmentation significative de l'activité de la Glucose-6-Phosphate-Dehydrogenase (G6PDH) pour la dose d'irradiation de 4.2 cGy (0.04 cGy/jour). Les deux types de contamination ont induit la diminution de l'incorporation de TdR dans les cellules de la moelle osseuse. Ces résultats suggèrent que la diminution de l'incorporation de TdR peut être liée à des modifications au niveau du transport transmembranaire pour des irradiations chronique de 4.2 cGy et aiguë de 5 cGy. Des phénomènes radioinduites intracellulaires sont probablement à l'origine de la diminution de l'incorporation de TdR pour les doses de 19.4 cGy et 20 cGy.

Key words: HTO, G6PDH, thymidine, internal contamination

© EDP Sciences 1999

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.