Free Access
J. Chim. Phys.
Volume 96, Number 1, January 1999
Page(s) 116 - 123
J. Chim. Phys. Vol. 96, N°1  p. 116-123
DOI: 10.1051/jcp:1999118

Antioxidant effect of naturally occurring xanthines on the oxidative damage of DNA bases

Vieira A.J.S.C.1, Telo J.P.1, Pereira H.F.1, Patrocínio P.F.1 and Dias R.M.B.2

1  Instituto Superior Técnico, Secção de Química Orgânica, Av. Rovisco Pais, P-1096 Lisboa Codex, Portugal
2  Instituto Tecnológico e Nuclear, Dep. de Química, Est. Nac. No. 10, P-2686 Sacavém Codex, Portugal

The repair of the oxidised radicals of adenine and guanosine by several naturally occurring xanthines was studied. Each pair of DNA purine/xanthine was made to react with the sulphate radical and the decrease of the concentration of both compounds was measured by HPLC as a function of irradiation time. The results show that xanthine efficiently prevents the oxidation of the two DNA purines. Theophyline and paraxanthine repair the oxidised radical of adenine but not the one from guanosine. Theobromine and caffeine do not show any protecting effect. An order of the oxidation potentials of all the purines studied is proposed.

La réparation des radicaux oxydés de l'adénine et de la guanosine par des xanthines naturelles a été étudiée en soumettant chaque paire base de l'ADN/xanthine à l'oxydation par le radical sulfate et en mesurant par HPLC la disparition des deux composés en fonction du temps d'irradiation. Les résultats montrent que la xanthine joue un rôle protecteur efficace contre l'oxydation des deux purines de l'ADN. La théophyline et la paraxanthine réparent le radical oxydé de l'adénine mais pas celui de la guanosine. La théobromine et la cafeíne n'ont pas d'effet protecteur. Un ordre de potentiels d'oxydation des purines étudiées est proposé.

Key words: antioxidants, DNA damage repair, oxidation potential, oxidised DNA bases, xanthines

© EDP Sciences 1999

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